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Recent Advances in Aptamer Discovery and Applications Yang Zhang1,* , Bo Shiun Lai2and Mario Juhas3 1College of Science, Harbin Institute of Technology, Shenzhen 518055, China 2School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA; [email protected] all results for this questionWhat kind of promoter is used to express Cas9?Normally, a DNA-dependent RNA polymerase II (Pol II) promoter and a Pol II transcriptional terminator are used to express Cas9 fused with a nuclear localization signal peptide, and a Pol III promoter and terminator is used to express the gRNA.See all results for this questionWhat are the recent advances in metabolic engineering?Provided a comprehensive and up-to-date review of the recent advances in metabolic engineering of Saccharomyces cerevisiae. Highlighted new tools for pathway optimization and genome engineering to advance metabolic engineering in yeast. Discussed the challenges and perspectives for high throughput metabolic engineering.See all results for this question
Recombinase polymerase amplification: Basics, applications recent advances in the applications of promoter
Recombinase polymerase amplification: Basics, applications and recent advances Trends Analyt Chem. 2018 Jan;98:19-35. doi: 10.1016/j.trac.2017.10.015. Epub 2017 Oct 26. Authors Ivan Magriñá Lobato 1 , Ciara K O'Sullivan 1 2 Affiliations 1 INTERFIBIO recent advances in the applications of promoterRecent advances of molecular toolbox construction expand recent advances in the applications of promoterNov 30, 2016 · In this mini-review, we addressed recent advances of molecular toolboxes, including synthetic promoters, signal peptides, and genome engineering tools that established for P. pastoris. Furthermore, the applications of P. pastoris towards synthetic biology were also discussed and prospected especially in the context of genome-scale metabolic pathway analysis.Cited by: 18Publish Year: 2017Author: Zhen Kang, Hao Huang, Yunfeng Zhang, Guocheng Du, Jian ChenRecent advances in the expression of foreign genes in recent advances in the applications of promoterAbstract. The Pichia pastoris heterologous gene expression system has been utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest. Recent advances in our understanding and application of the system have improved its utility even further. These advances include: (1) methods for the construction of P. pastoris strains with multiple copies of AOX1-promoter Cited by: 1385Publish Year: 1993Author: James M. Cregg, Thomas S. Vedvick, William C. Raschke
Recent advances in the applications of promoter engineering for the optimization of metabolite biosynthesis Cell metabolism in living organisms is largely regulated at the transcriptional level, and the promoters are regarded as basic regulatory elements responsible for transcription initiation.Cited by: 21Publish Year: 2019Author: Ning Xu, Liang Wei, Jun LiuRecent advances in the applications of promoter recent advances in the applications of promoterIn this review, we will briefly introduce the architecture and function of both prokaryotic and eukaryotic promoters, and provide an overview of several major approaches for promoter engineering. The recent achievements and advances by promoter engineering for the optimization of metabolite biosynthetic pathways in multiple widely-used model microorganism, including Escherichia coli, Corynebacterium Recent advances in the applications of promoter recent advances in the applications of promoterCited by: 21Publish Year: 2019Author: Ning Xu, Liang Wei, Jun LiuAbstract. Cell metabolism in living organisms is largely regulated at the transcriptional level, and Introduction. Metabolic engineering is a powerful tool for the design, construction, modification and
Recent advances in the application of multiplex genome recent advances in the applications of promoter
Apr 27, 2021 · Using this approach, promoters at different positions can be edited simultaneously, with 68% efficiency for editing 6 promoter and 32% efficiency for 8 promoters. This result is encouraging; further investigation are needed in genes editing, especially the longer gene fragment. recent advances in the applications of promoter Recent advances in the application of multiplex genome editing recent advances in the applications of promoterRecent advances in recombinant protein expression by recent advances in the applications of promoterSep 26, 2013 · Among constitutive promoters, the ermE promoter is a strong promoter and among the most widely used promoters for protein expression in Streptomyces (Bibb et al. 1985; Strohl 1992). For example, the ermE promoter increases the yield of tendamistat, an -amylase inhibitor, up to 500 mg/L (Schmitt-John and Engels 1992).Cited by: 21Publish Year: 2013Author: Long Liu, Haiquan Yang, Hyun-dong Shin, Jianghua Li, Guocheng Du, Jian ChenRecent advances in recombinant adeno-associated virus recent advances in the applications of promoteruse of this vector system. This review discusses the recent rep-inducible p40 cap gene promoter. Finally, because advances in vector production technology that have re-AAV and recombinant derivatives are replication defec-sulted in the routine isolation of 104 recombinant viral tive, these viruses require helper virus gene products particles recent advances in the applications of promoter
Recent advances in metabolic engineering of Saccharomyces recent advances in the applications of promoter
Nov 01, 2018 · The control of gene expression is mainly achieved via promoter engineering, while the role of terminators has been less explored in yeast. Curran et al. characterized over 30 terminators in the context of multiple promoters (Curran et al., 2013a).Cited by: 124Publish Year: 2018Author: Jiazhang Lian, Shekhar Mishra, Huimin ZhaoRecent advances in in vivo applications of intein-mediated recent advances in the applications of promoterFeb 04, 2014 · Recent work has extended these in vitro applications to the cell or to whole organisms. We review recent advances in intein-mediated protein expression and modification, post-translational processing and labeling, protein regulation by conditional protein splicing, biosensors, and expression of Recent advances in genetic engineering tools based on recent advances in the applications of promoterJan 02, 2020 · Recently developed strategies, such as clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9, multiplex automated genome engineering (MAGE), promoter engineering, CRISPR-based regulations, and synthetic small regulatory RNA (sRNA)-based knockdown, are considered as powerful tools for genome-scale engineering in microbiological systems.
Apr 12, 2021 · Recent advances in the development of high-throughput selection and screening systems. Selection systems have become standardized during the decades, however, the advantages versus disadvantages of different selection systems continue to form a matter of debate, especially in the case of the MTX selection system.Author: Borbála Tihanyi, László NyitrayPublish Year: 2021Recent Advances of In Vitro Culture for the Application of recent advances in the applications of promoterDespite the many advances that have been made in the last years (including the use of inducible promoters and site-specific recombination systems), transformation efficiency, and regeneration potential still represent a bottleneck in the application of the new Cited by: 2Publish Year: 2020Author: Lara Poles, Concetta Licciardello, Gaetano Distefano, Elisabetta Nicolosi, Alessandra Gentile, Stefa recent advances in the applications of promoterEstimated Reading Time: 3 minsRecent Advances in Genetic Technique of Microbial Report recent advances in the applications of promoterMicrobial cell arrays have attracted consistent attention for their ability to provide unique global data on target analytes at low cost, their capacity for readily detectable and robust cell growth in diverse environments, their high degree of convenience, and their capacity for multiplexing via incorporation of molecularly tailored reporter cells. To highlight recent progress in the field of microbial cell arrays, this review discusses research on genetic engineering of reporter cells, technologies for patterning live cellSee more on hindawi recent advances in the applications of promoterCited by: 1Publish Year: 2015Author: Do Hyun Kim, Moon Il Kim, Hyun Gyu Park
Unqualified welding quality of recent advances in the applications of promoter is prone to hidden dangers such as oil tank steel plate cracks and leakage. To avoid this situation as much as possible, it needs to be avoided when the oil storage tank is manufactured. Scientific and reasonable measures are adopted to control the welding quality when welding the recent advances in the applications of promoter. How to reasonably control the welding process quality of oil storage tanks?